Background

This project investigates the fate of cyanogenic glycosides (CNGs) in plant-based foods and their potential to release hydrogen cyanide (HCN) during processing, chewing, and digestion. Using biochemical, enzymological, and analytical chemistry approaches (FPLC, proteomics, LC-MS), it will characterise enzymes involved in CNG hydrolysis. Simulated gastrointestinal digestion models will assess HCN release and stability, with uptake studies using CaCo2 cell models. The findings will enhance understanding of CNG metabolism, HCN bioavailability, and associated health risks, supporting food safety and public health assessments.

The Role

We are seeking a highly motivated Postdoctoral Researcher with expertise in biochemistry, enzymology, and analytical chemistry to join our research team on a twelve-month basis. The role will focus on investigating enzyme activity in biological and food systems, utilising advanced chromatographic, spectrometric, and proteomic techniques to characterise biochemical processes.

The successful candidate will apply their expertise in enzyme purification, fractionation, and characterisation, working with a range of biochemical and analytical approaches, including LC-MS, FPLC, and proteomics. The research will involve simulated biological processes to assess enzymatic transformations and compound stability. The role will also include data analysis, interpretation, and reporting to contribute to scientific understanding in this field.

Key Responsibilities

• Purify and characterise enzymes from biological and food sources using chromatographic and proteomic techniques.
• Conduct biochemical and enzymatic assays to assess enzyme activity, specificity, and stability.
• Investigate metabolic and enzymatic pathways in simulated biological conditions.
• Apply spectrometric and chromatographic methods to analyse enzyme-substrate interactions.
• Develop and use in vitro digestion and fermentation models to study enzymatic degradation and compound stability.
• Process, analyse, and interpret biochemical and enzymatic data.
• Prepare technical reports, peer-reviewed publications, and present research findings.
• Collaborate with multidisciplinary teams and ensure compliance with laboratory safety and research ethics.

About the Research Group

The Kroon group has been at the forefront of biochemical and nutritional research for over 20 years, focusing on the interactions between enzymes, phytochemicals, and human biology. Their work spans analytical chemistry, enzymology, microbiology, and human nutrition, utilising chromatographic, spectrometric, and cell-based approaches to investigate the role of bioactive compounds in health and disease. The group employs simulated digestion models, in vitro fermentation techniques, and biochemical assays to characterize metabolic and enzymatic processes.

Required Skills and Experience

• Four years’ experience and PhD in biochemistry, enzymology, analytical chemistry, or a related field.
• Expertise in enzyme purification, characterisation, and biochemical assays.
• Strong experience with chromatographic (LC-MS, FPLC) and proteomic techniques.
• Familiarity with simulated biological processes and enzymatic transformations.
• Proficiency in data analysis and interpretation of biochemical experiments.
• Strong scientific writing and reporting skills.
• Ability to work both independently and as part of a multidisciplinary team.

How to Apply

To apply for this vacancy, please send a copy of your CV along with a brief covering letter and contact details for two references to [contact hidden]. The closing date for applications is 28 March 2025.